Journal: Immunity

Article Title: SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2

doi: 10.1016/j.immuni.2021.05.006

Figure Lengend Snippet:

Article Snippet: Human CD299, hFc-tagged , Sino Biological , Cat#: 10559-H01H.

Techniques: Staining, Recombinant, Enzyme-linked Immunosorbent Assay, Western Blot, SYBR Green Assay, Multiplex Assay, Infection, shRNA, cDNA Library Assay, Software, Luminex

Journal: Genes

Article Title: Association Between Single-Nucleotide Polymorphism rs2287886 of CD209 Gene and Clinical Severity of COVID-19 in Unvaccinated Brazilian Patients

doi: 10.3390/genes16091029

Figure Lengend Snippet: eQTL violin plot of rs2287886 variant. The genotype of rs2287886 was associated with gene expression of CD209 gene in different tissues in GTEx database. ( a ) Esophagus, ( b ) adipose, ( c ) artery, and ( d ) esophagus.

Article Snippet: CD209 and CLEC4M are closely related in sequence and function, thus indicating a coordinated role in the immune response [ ] (The Human Protein Atlas).

Techniques: Variant Assay, Gene Expression

Journal: Genes

Article Title: Association Between Single-Nucleotide Polymorphism rs2287886 of CD209 Gene and Clinical Severity of COVID-19 in Unvaccinated Brazilian Patients

doi: 10.3390/genes16091029

Figure Lengend Snippet: A protein–protein interaction network for the CD209 gene showing 11 nodes. The network was visualized using STRING, with edge color intensity indicating the confidence level of functional associations.

Article Snippet: CD209 and CLEC4M are closely related in sequence and function, thus indicating a coordinated role in the immune response [ ] (The Human Protein Atlas).

Techniques: Functional Assay

Journal: Journal of Cancer

Article Title: CLEC4M is associated with poor prognosis and promotes cisplatin resistance in NSCLC patients

doi: 10.7150/jca.30139

Figure Lengend Snippet: CLEC4M upregulates the protein expression of XPA and ERCC1 in A549 and H1299 cells. (A-D) XPA, RAD23B, ERCC1 and XPA mRNA levels in CLEC4M-silenced A549 and H1299 cells (A and B) or CLEC4M-overexpressing A549 and H1299 cells (C and D) were measured by RT-qPCR. (E-H) XPA, RAD23B, ERCC1 and XPA protein expression in CLEC4M-silenced A549 and H1299 cells (E and F) or CLEC4M-overexpressing A549 and H1299 cells (G and H). Data are presented as the mean ± SD, * p < 0.01, ** p < 0.01, *** p < 0.01 vs. shCon or vector.

Article Snippet: The antibodies used were as follows: β-actin (1:5000; Proteintech), CLEC4M (1:1000; Abcam), Caspase-3 (1:1000; CST), Cleaved Caspase-3 (1:1000; CST), XPA (1:1000; Proteintech), RAD23B (1:1000; Proteintech), XPG (1:1000; Proteintech), and ERCC1 (1:1000; Proteintech).

Techniques: Expressing, Quantitative RT-PCR, Plasmid Preparation

Journal: Journal of Cancer

Article Title: CLEC4M is associated with poor prognosis and promotes cisplatin resistance in NSCLC patients

doi: 10.7150/jca.30139

Figure Lengend Snippet: CLEC4M upregulates the protein expression of XPA and ERCC1 in A549 and H1299 cells. (A-D) XPA, RAD23B, ERCC1 and XPA mRNA levels in CLEC4M-silenced A549 and H1299 cells (A and B) or CLEC4M-overexpressing A549 and H1299 cells (C and D) were measured by RT-qPCR. (E-H) XPA, RAD23B, ERCC1 and XPA protein expression in CLEC4M-silenced A549 and H1299 cells (E and F) or CLEC4M-overexpressing A549 and H1299 cells (G and H). Data are presented as the mean ± SD, * p < 0.01, ** p < 0.01, *** p < 0.01 vs. shCon or vector.

Article Snippet: The antibodies used were as follows: β-actin (1:5000; Proteintech), CLEC4M (1:1000; Abcam), Caspase-3 (1:1000; CST), Cleaved Caspase-3 (1:1000; CST), XPA (1:1000; Proteintech), RAD23B (1:1000; Proteintech), XPG (1:1000; Proteintech), and ERCC1 (1:1000; Proteintech).

Techniques: Expressing, Quantitative RT-PCR, Plasmid Preparation

Journal: Journal of Cancer

Article Title: CLEC4M is associated with poor prognosis and promotes cisplatin resistance in NSCLC patients

doi: 10.7150/jca.30139

Figure Lengend Snippet: CLEC4M upregulates the protein expression of XPA and ERCC1 in A549 and H1299 cells. (A-D) XPA, RAD23B, ERCC1 and XPA mRNA levels in CLEC4M-silenced A549 and H1299 cells (A and B) or CLEC4M-overexpressing A549 and H1299 cells (C and D) were measured by RT-qPCR. (E-H) XPA, RAD23B, ERCC1 and XPA protein expression in CLEC4M-silenced A549 and H1299 cells (E and F) or CLEC4M-overexpressing A549 and H1299 cells (G and H). Data are presented as the mean ± SD, * p < 0.01, ** p < 0.01, *** p < 0.01 vs. shCon or vector.

Article Snippet: The antibodies used were as follows: β-actin (1:5000; Proteintech), CLEC4M (1:1000; Abcam), Caspase-3 (1:1000; CST), Cleaved Caspase-3 (1:1000; CST), XPA (1:1000; Proteintech), RAD23B (1:1000; Proteintech), XPG (1:1000; Proteintech), and ERCC1 (1:1000; Proteintech).

Techniques: Expressing, Quantitative RT-PCR, Plasmid Preparation